Table 1.
Plasmids used in this study
| Vectors | Description | Source |
|---|---|---|
| pENTR3C | Gateway entry vector | Invitrogen |
| pDONR207 | Gateway donor vector | Invitrogen |
| pDest15 | Bacterial GST fusion expression vector; T7 promoter | Invitrogen |
| pGEX-4T-1 | Bacterial GST fusion expression vector; tac promoter | Amersham |
| pDest53 | Mammalian GFP fusion expression vector; CMV and T7 promoter | Invitrogen |
| pDestTH1 | Bacterial MBP fusion expression vector; tac promoter | [59] |
| pDestEGFP-C1 | Mammalian EGFP fusion expression vector; CMV promoter | [60] |
| pDestMyc | Mammalian Myc fusion expression vector; CMV and T7 promoter | [60] |
| pDestHA | Mammalian HA fusion expression vector; CMV promoter | [60] |
| pGEX-KG-SKS-Sp1(DBD) | Bacterial GST fusion expression vector; tac promoter | [61] |
| pGEX-KG-SKS-BTEB1(DBD) | Bacterial GST fusion expression vector; tac promoter | [61] |
| pGEX-KG-SKS-Sp3(DBD) | Bacterial GST fusion expression vector; tac promoter | [61] |
| pGEX-KG-SKS-BTEB2(DBD) | Bacterial GST fusion expression vector; tac promoter | [61] |
| p21-Luc | [56] | |
| pDestMyc-PKCλ | Murine PKCλ in entry vector | [60] |
| cDNA constructs made by traditional cloning or site directed mutagenesis | ||
| pDONR207-TIEG1 | Human TIEG1(1–480) in donor vector | This study |
| pENTR3C-TIEG1(1–362) | Human TIEG1(1–362) in entry vector | This study |
| pENTR3C-TIEG1(DBD) | Human TIEG1(363–480) in entry vector | This study |
| pENTR3C-Sp1 | Human Sp1(1–785) in entry vector | This study |
| pENTR3C-KLF4 | Human KLF4 (1–470) in entry vector | This study |
| pENTR3C-KLF4(DBD) | Human KLF4 (381–470) in entry vector | This study |
| pENTR3C-Egr1 | Human Egr1(1–543) in entry vector | This study |
| pENTR3C-TIEG1(1–362)Sp1(DBD) | Human TIEG1(1–362)Sp1(619–714) in entry vector | This study |
| pENTR3C-TIEG1(DBD)S384A | Human TIEG1(363–480) with S384A point mutation in entry vector | This study |
| pENTR3C-TIEG1(DBD)T445A | Human TIEG1(363–480) with T445A point mutation in entry vector | This study |
| pENTR-TIEG1(DBD)S384A/T445A | Human TIEG1(363–480) with S384A/T445A point mutations in entry vector | This study |
| pENTR3C-TIEG1(DBD)S384E | Human TIEG1(363–480) with S384E point mutation in entry vector | This study |
| pENTR3C-TIEG1(DBD)T445E | Human TIEG1(363–480) with T445E point mutation in entry vector | This study |
| pENTR3C-TIEG1(DBD)S384E/T445E | Human TIEG1(363–480) with S384E/T445E point mutations in entry vector | This study |
| pDONR207-TIEG1S384A | Human TIEG1 with S384A point mutation in donor vector | This study |
| pDONR207-TIEG1T445A | Human TIEG1 with T445A point mutation in donor vector | This study |
| pDONR207-TIEG1S384A/T445A | Human TIEG1 with S384A/T445A point mutations in donor vector | This study |
| pDONR207-TIEG1S384E | Human TIEG1 with S384E point mutation in donor vector | This study |
| pDONR207-TIEG1T445E | Human TIEG1 with T445E point mutation in donor vector | This study |
| pDONR207-TIEG1S384E/T445E | Human TIEG1 with S384E/T445E point mutations in donor vector | This study |
| pGEX-KG-SKS-Sp1(DBD)S641A | Human Sp1(DBD) with S641A point mutation in expression vector | This study |
| pGEX-KG-SKS-Sp1(DBD)S702A | Human Sp1(DBD) with S702A point mutation in expression vector | This study |
| pGEX-KG-SKS-Sp1(DBD)S641A/S702A | Human Sp1(DBD) with S641A/S702A point mutations in expression vector | This study |
| pGEX-KG-SKS-Sp1(DBD)S641E/S702E | Human Sp1(DBD) with S641E/S702E point mutations in expression vector | This study |
| pENTR-PKCλ reg | Murine PKCλ (1–247) in entry vector | This study |
| cDNA constructs made by Gateway LR reaction (this study) | ||
| pDestMyc-PKCλ | pDest15-TIEG1(DBD)S384A | pDest53-TIEG1(DBD) |
| pDestGBKT7-PKCλ reg | pDest15-TIEG1(DBD)T445A | pDestHA-TIEG1 |
| pDestEGFP-TIEG1S384A | pDest15-TIEG1(DBD)S384A/T445A | pDestHA-KLF4 |
| pDestEGFP-TIEG1T445A | pDest15-TIEG1(DBD)S384E | pDestHA-Sp1 |
| pDestEGFP-TIEG1S384A/T445A | pDest15-TIEG1(DBD)T445E | pDest3X-FLAG-Sp1 |
| pDestEGFP-TIEG1S384E | pDest15-TIEG1(DBD)S384E/T445E | pDestMyc-TIEG1(1–362)Sp1(DBD) |
| pDestEGFP-TIEG1T445E | pDestTH1- TIEG1 | pDestMyc-KLF11 |
| pDestEGFP-TIEG1S384E/T445E | pDestTH1- TIEG1S384A | pDestHA-KLF11 |
| pDest15-TIEG1 | pDestTH1- TIEG1T445A | pDestEGFP-EGR1 |
| pDest15-TIEG1(1–362) | pDestTH1- TIEG1S384A/T445A | |
| pDest15-TIEG1(DBD) | pDest15-KLF4(DBD) | |