Figure 3.

Reducing activity reduces the number of functional synapses formed in ABNs. (A) Total spines per cell (gray dots) and group means (bars with SE) for reconstructed ABNs. Both siRNA knock-down and naris occlusion showed significant reductions in total numbers of spines compared to controls (control/siRNA: p = 0.031, n = 20; control/NO: p = 0.003, Wilcoxon Rank-Sum test, n = 21) in the number of formed synapses. (B,C) Distribution of spines plotted as total number of spines per 40 μm bin vs. distance along apical dendrite path with SE. Significant differences occurred in early segments (40–80 μm) of apical dendrites in siRNA knock-down cells (B); (p < 0.05 and p < 0.05 respectively, n = 20).