Figure 7.

CBX7 modulates the miR-181b expression. MirVana qRT-PCR analysis of miR-181a and miR-181b expression in MEFs^−/− or ^+/− for CBX7 gene. The relative amount of the RNA was normalized to the U6 RNA using the 2^−ΔCt method,²⁵ and the fold-change values indicated the relative change in expression levels between the CBX7^−/− or CBX7^+/− versus wild-type cells, assuming that the value of wild-type MEFs was equal to 1. The results are reported as the mean of the expression values, with error bars indicating standard deviation (mean ± SD); n = 3. In the bottom, we show the Western blot analysis of CBX7 expression in MEFs wild type, MEFs^−/−, or ^+/− for CBX7.