Figure 8.

The miR-181b, HMGA1, and CBX7 protein expression in human breast neoplasias. (A) Immunohistochemical analysis of HMGA1 and CBX7 expression in malignant breast neoplasias. Paraffin sections from neoplastic breast tissues were analyzed by immunohistochemistry with antibodies raised against specific antibodies against a specific HMGA1 and CBX7 peptide. No immunoreactivity with HMGA1 antibody appeared in normal tissue (a), while nuclear staining is evident in G1 ductal carcinoma (b), and more intense staining is present in G3 ductal (c) and lobular breast carcinomas (d). CBX7 nuclear staining was intense in normal breast tissue (e), while the decrease of CBX7 in neoplastic lesions was progressive going from well-differentiated cancer, such as from G1 (f) to G3 ductal carcinoma (g), but in particular, no immuoreactivity appeared in lobular breast carcinoma (h). (B) MirVana qRT-PCR analysis of miR-181b expression. The fold-change values indicate the relative change in the expression levels between normal and carcinoma samples, assuming that the value of each matched normal breast tissue is equal to 1.