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. 2010 Mar;1(3):210–224. doi: 10.1177/1947601910366860

Figure 9.

Figure 9.

HMGA proteins modulate CBX7 expression in vitro and in vivo. (A) RT-PCR analysis of CBX7 mRNA expression in rat thyroid cells expressing or not hmga1 gene. GAPDH gene expression was evaluated as control to normalize the amount of the used RNAs. (B) Soluble chromatin from FRTL5 and FRTL5-KiMSV was immunoprecipitated with anti-HMGA antibody, and the DNAs were amplified by PCR. IgG was used as immunoprecipitation control. (C) qRT-PCR analysis of HMGA1b mRNA expression. The fold-change values indicate the relative change in the expression levels between normal and carcinomas samples, assuming that the value of each matched normal breast tissue is equal to 1. (D) qRT-PCR analysis of CBX7 mRNA expression in the samples shown in panel C. (E) Plot of the data generated from panels C and D defines the linear relationship between HMGA1b and CBX7 expression by measuring the Pearson coefficient correlation reported in the figure. (F) Novel pathway leading to cancer progression. The induction of HMGA1 down-regulates CBX7 expression and up-regulates miR-181b, which in turn would further decrease CBX7 protein level.