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Roscovitine and CR8 induce massive down-regulation of Mcl-1 mRNA. SH-SY5Y NB cells were exposed for various durations to roscovitine or CR8 at either a (A) 50-µM or (B) 5-µM final concentration. Total mRNA was extracted, and reverse-transcribed. Mcl-1 (136 bp fragment) was then amplified by PCR (28 cycles), and the reaction products were resolved by electrophoresis on agarose. Expression of actin mRNA (233-bp fragment) was used as an internal control.
