Figure 3.

Roscovitine and CR8 induce massive down-regulation of Mcl-1 protein. (A) SH-SY5Y cells were exposed to increasing concentrations of roscovitine or CR8 for 48 hours, and proteins were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting with anti-Mcl-1 antibodies. Lower concentration scale applies to CR8; upper scale applies to roscovitine. Etp = etoposide. Arrow indicates a low molecular weight degradation product of Mcl-1. (B) CR8 triggers Mcl-1 protein down-regulation in 9 human NB cell lines. NB cell lines were exposed to various concentrations of CR8 for 24 hours and processed as described above.