Figure 4.

Down-regulation of Mcl-1 is not induced by inhibitors of other kinase targets of roscovitine or CR8. SH-SY5Y cells were exposed for 10 hours to pharmacological inhibitors of DYRK1A (Made44, harmine), CK1 (IC261, DRF053, D4476), CLK1 (TG003), MEK1 (UO126, PD098059); to a general kinase inhibitor (staurosporine); to a kinase-inactive derivative of roscovitine (N6-methyl-roscovitine); or to roscovitine or CR8. All drugs were tested at 5, 15, and 50 µM, except staurosporine (0.2, 0.6, and 2 µM). Cells were harvested and proteins were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting with anti-Mcl-1 antibodies. α-Tubulin was used as a loading control.