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. 2010 Sep;1(9):883–892. doi: 10.1177/1947601910389605

Figure 4.

Figure 4.

TPPII localizes to the centrosome and stimulates centriole multiplication. (A) Immunofluorescence microscopic analysis of TPPII in U-2 OS/centrin-GFP cells. Note the presence of TPPII at centrioles throughout the cell division cycle. Nuclei stained with DAPI. Scale bar indicates 10 µm. (B) Fluorescence microscopic analysis of centrioles in a U-2 OS/centrin-GFP cells transiently transfected with empty vector (control) or TPPII. Note the presence of centriole multiplication in the TPPII-transfected cell as evidenced by the concurrent presence of 2 daughter centrioles (arrows) at a single maternal centriole (arrowhead). (C) Quantification of centriole overduplication (gray bars) and centriole multiplication (black bars) in U-2 OS/centrin-GFP cells transiently transfected with empty vector (control) or TPPII. Each bar indicates average ± standard error of 3 independent experiments with at least 100 cells counted per experiment. Asterisks indicate statistically significant differences and P values.