Figure 5.
Re-expression of MCC in HCT15 cells slows proliferation rate and induces G1/S and G2/M arrest. (A) Stable MCC-expressing and control HCT15-derived clones were generated using the pDEST-MT vector system. HCT15 stably expressing MCC cells and control cells were seeded at 5.104 cells at day 0. Cells were harvested and counted in triplicate over a 7-day time course. Graphed values are the average number of cells counted ± SEM. (B) FACS analysis was performed at day 3 and day 5 of the growth curve. (C) Western blot shows MCC expression in the stable cell lines after 1 passage (1) and 2 passages (2). (D) Total lysates of cells harvested at day 5 were blotted for MCC, cyclin B1, and GAPDH (LC).