Table 1.

Summary of the Analyses of Clinical Specimens for Crk and/or miR-126 Expression in Human Cancers

Cancer Type: Lung Cancer (Miller et al.30)
Classification	Normal	Stage I	Stage III
Total Crk mRNA (sample size)	CNA	+ (67)	++ (19)
Crk protein levels
CrkI	+	++	+++
CrkII	+	+	+
pCrkII	+	++	++
Sample size	10	57	19
Classification	Well Differentiated	Moderately Differentiated	Poorly Differentiated
Total Crk mRNA	+	+	++
Crk protein levels
CrkI	+	+	++
CrkII	+	++	+++
pCrkII	+	++	+++
Sample size	22	47	23
Cancer Type: Breast Cancer (Rodrigues et al.32)
Classification	Normal	Stage I	Stage III
No. of samples	—a	8	12
Crk protein level elevated	—a	63%	58%
Cancer Type: Glioblastoma (Wang et al.41)
Classification	Normal Brain Tissue	Benign (Grade I, II)	Malignant (Grade III, IV)
Crk mRNA level
CrkI	+	+	++ (8 cases)
CrkII	+	+	++ (3 cases)
Sample size	1	4	16
Cancer Type: Gastric Cancer (Feng et al.58)
Classification	Local Invasion: T1, T2	Local Invasion: T3, T4
miR-126 expression (n = 60)	Low (7 cases), high (15 cases)	Low (26 cases), high (12 cases)
Classification	Lymph Node Metastasis: No	Lymph Node Metastasis: Yes
miR-126 expression (n = 60)	Low (6 cases), high (13 cases)	Low (27 cases), high (14 cases)
Classification	TNM Stage I, II	TNM Stage III, IV
miR-126 expression (n = 60)	Low (4 cases), high (18 cases)	Low (29 cases), high (9 cases)

Note: Crk mRNA levels were analyzed either by Affymetrix oligonucleotide arrays (in lung cancer) or semiquantitative RT-PCR (in glioblastoma), and Crk protein levels were analyzed either by 2D-PAGE and Western blotting (in lung cancer) or immunohistochemistry (lung and breast cancer). miR-126 expression was analyzed by qRT-PCR. CNA = comparison not available; TNM = tumor node metastasis.

^aStaining of adjacent normal epithelia was used for comparison.