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. 2011 May 9;208(5):1093–1103. doi: 10.1084/jem.20101527

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Figure 3. — IL-4 is both essential and sufficient to sustain memory-like CD8⁺ T cells in BALB/c thymus. (A) Expression of Eomes after culture of thymocytes from wild-type and Klf13^−/− BALB/c mice with IL-4, IL-7, IL-15, or medium. (B) Eomes expression in CD8⁺ SP cells from thymi of Klf13^−/− BALB/c mice after injection with 1 µg IL-4 immune complex or vehicle every other day for 10 d. (C) Relative abundance of IL-4, IL-7, or IL-15 mRNA relative to HPRT1 in thymi from the indicated stains of mice as measured by real-time RT-PCR. Error bars are SDs from triplicate PCR. (D) IL-4 and TCRβ expression in thymi from the indicated strains of mice after treatment with ionomycin and PMA in the presence of brefeldin A for 4 h. (E) FACS analysis of the expression of interferon-γ and IL-4 in wild-type and Il4^−/− thymus from BALB/c mice. Thymocytes were treated with ionomycin and PMA in the presence of brefeldin A for 4 h before analysis. (F) Expression of Eomes, IFN-γ, and CD24 in CD8⁺ SP thymocytes from wild-type and Il4^−/− BALB/c mice. Data are representative of two (A and C) or three (B–F) independent experiments with two (B) or three (D, E, and F) mice in each genotype group.