Figure 5.

DKO mice exhibit reduced OVA-specific IgE production and attenuated nasal symptoms. (A, B, and C) ELISA for OVA-specific IgE (A) and IgG (B) in serum and IgA in nasal wash (C). WT and DKO mice were immunized intranasally with OVA and CT in PBS (closed and open triangles, respectively) or with PBS alone (closed and open circles, respectively) on days 0, 7, and 14, and OVA-specific antibodies were measured by ELISA on day 21. Error bars represent the SD of triplicate determinations. *, P < 0.05; **, P < 0.01. Data are representative of four independent experiments. (D) Nasal symptoms after intranasal immunization. WT and DKO mice were intranasally immunized with OVA and CT on days 0, 7, and 14. On day 21, mice were intranasally challenged with PBS alone or OVA and CT in PBS. 2 min later, the number of sneezes was counted for 5 min. Each horizontal bar represents the mean of the values obtained from 6–10 animals. *, P = 0.07. Data from three separate experiments were pooled.