Figure 7. vATPase inhibition does not affect CER (100nM) dependent changes in cell morphology.
Acini were incubated with the vATPase inhibitors concanamycin-A (100nM), lobatomide-B(1μM) or salicylihalamide-A(1μM) for 1 hour prior to CER(100nM) treatment for an additional hour. Cells were then embedded and assessed for cytosolic vacuole formation and membrane blebing. A=Control, B=CER(100nM), C=CER+Concanamycin, D=CER+Labatomide and E=CER+Salicylihalamide. Arrows indicate membrane blebing and arrowheads indicate vacuoles. Images were taken at 40X magnification and are representative of each treatment group.