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. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Circ Res. 2008 Mar 20;102(9):1127–1136. doi: 10.1161/CIRCRESAHA.107.163642

Figure 3. Alteration of FAK downstream signaling induced by MR is prevented by β1-RB.

Figure 3

Figure 3

(A) FAK immunoprecipitates from LV lysates of control, 4W-MR, and 4W-MR+β1-RB dogs were resolved on SDS-PAGE and blotted with anti-phosphotyrosine, p130Cas, paxillin, or FAK antibodies. (B–C) p130Cas or paxillin immunoprecipitates from control, 4W-MR, or 4W-MR+β1-RB LV extracts were resolved on SDS-PAGE and blotted with anti-phosphotyrosine, anti-p130Cas (B), or anti-paxillin (C) antibodies. Top, representative autoradiograms (with each lane from a single gel exposed for the same duration). Bottom, fold induction, n=6 each group, *P<0.05 4W-MR vs. control, #P<0.05 4W-MR+β1-RB vs. 4W-MR. (D) Representative immunoblot showing accumulation of phospho-Pyk2 Tyr-402 in LV extracts from control, 4W-MR, and 4W-MR+β1-RB. Blot was stripped and blotted with anti-Pyk2 antibodies.