A, RT-PCR data showing a decrease in the SLUG transcript level in SLUG knocked down (SLUG siRNA) resting BT-549 cells over that in the control siRNA-treated cells. B, a Western blot showing a decrease in the SLUG protein level in SLUG knocked down (SLUG siRNA) BT-549 cells over that in the control siRNA-treated cells. C, inhibition of the function of the BRCA2 gene silencer in the SLUG knocked down (siRNA) resting BT-549 cells. Data are presented as mean (n = 12) ± S.E. The differences between the luciferase activities in the cells (transfected with the pGL3-PS construct) treated with control siRNA and SLUG siRNA were statistically significant (p < 0.0005). RLU, relative light units. D, RT-PCR data showing a severalfold increase in the BRCA2 mRNA level in the anti-SLUG siRNA-treated resting BT-549 cells over that in the control siRNA-treated cells. A parallel evaluation of β-actin mRNA was used as a loading control for the RT-PCR assays.