Figure 1. Tissue preparation to single cells.

(a) Tissue is marked up by the Pathology core laboratories. (b) Marked edges of the tissue are removed manually using scissors (inset shows trimmed edges), (c) and the remaining unmarked tissue is weighed. (d) Tissue is transferred to a tissue culture dish to be cut into small fragments. (e) After overnight digestion, samples contain red blood cells (left) that can be lysed to leave remaining cells (right). (f) Single dissociated cells can be counted by hemacytometer.