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Antibodies used to identify and isolate distinct epithelial and immune cell subsets
| Antigen | Conjugate (Company) | Volume (μl) for Single Antibody Controls (1 × 10E4 cells) | Volume (μl) for Sorted Sample (per 1 × 10E6 cells/100 (μl) |
|---|---|---|---|
| CD49f | PE (eBiosciences) | 1 | 3 |
| Alexa647 (BioLegend) | 1 | 3 | |
| Trop2 | APC (R&D) | 3 | 10 |
| CD24 | Biotin (StemCell Technologies) | 5 | 15 |
| Streptavidin | FITC (BD) | 1 | 5 |
| CD45 | APC-eFluor780 (eBiosciences) | 1 | 5 |
| CD8 | PE-cy7 (eBiosciences) | 1 | 5 |
