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The C2A hydrophobic mutations do not disrupt synaptotagmin expression or targeting. A, Representative Western blots from P[sytA-FY] line 3, line 5, and P[sytA-FE] and their P[sytWT] controls. B, P[sytA-FY] line 5 (n = 17) expressed less transgenic protein than P[sytWT] (n = 28) and the other two C2A mutants (P[sytA-FY] line 3, n = 7; P[sytA-FE], n = 11; p < 0.001). C, Transgenic synaptotagmin was localized to synaptic sites in all lines (line 5 shown for P[sytA-FY]). Scale bar, 20 μm.
