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Apoptotic cell death and modification of C5′ bulk. (A and B) Apoptotic cell death (%) was compared for each C5′-alkyl substituted digitoxin rhamnoside and amicetoside at 50 nM concentration (one-way ANOVA; N = 6; ***, P < 0.001). (C and D) Hoechst 33342 stained apoptotic cells appear in blue and propidium iodide stained necrotic cells in red at 50 nM drug concentration.
