Table 1. Cytotoxicity Evaluation of Digitoxin Analogues on NCI-H460 Human Lung Cancer Epithelial Cells.
| compd | IC50a | GI50b | compd | IC50a | GI50b |
|---|---|---|---|---|---|
| α-l-rhamnoside | α-l-amicetoside | ||||
| C5′-Me-4 | 52 ± 1 | 2 ± 1 | C5′-Me-9 | 52 ± 1 | 2 ± 1 |
| C5′-Et-5 | 57 ± 1 | 2 ± 1 | C5′-Et-10 | 87 ± 1 | 2 ± 1 |
| C5′-n-Pr-6 | 116 ± 1 | 5 ± 1 | C5′-i-Pr-11 | 533 ± 1 | 8 ± 1 |
| C5′-i-Pr-7 | 130 ± 1 | 6 ± 1 | C5′-i-Bu-12 | 458 ± 1 | 8 ± 1 |
| C5′-i-Bu-8 | 180 ± 1 | 13 ± 1 | |||
a
The IC50 value was measured by a 12 h treatment in Hoechst and propidium iodide stain assays.
b
The GI50 value was measured by a 48 h treatment in an MTT assay. All values represent the standard error of the mean value of three independent experiments with duplicate determinations.