Table 5.
Sequence and characteristics of conserved miRNAs in P. olivaceus.
| miRNA family | miRNA name | No. of clones | Sequence (5′-3′) | Length (bp) | Conserved in other animals |
|---|---|---|---|---|---|
| pol-miR-1 | pol-miR-1a | 1 | UGGAAUGUAAAGAAGUAUGUA | 21 | ssc, cfa, mmu-miR-1-2-as, mdo, xtr, ppa, gga, cbr, cel |
| pol-let-7 | pol-let-7a | 3 | UGAGGUAGUAGGUUGUAUAGUU | 22 | eca, bfl-let-7-1-as, sko, lgi, bfl, ptr, cfa, mml, mdo, bta, xtr, tni, fru, dre, gga, rno, mmu, cbr, hsa, cel |
| pol-let-7e | 2 | UGAGGUAGUAGAUUGAAUAGUU | 22 | oan, tni, fru, dre, gga | |
| pol-miR-7f | 1 | UGAGGUAGUAGAUUGUAUAGUU | 22 | eca, ptr, cfa, mml, oan, mdo, xtr, bta, ssc, dre, gga, rno, mmu, | |
| pol-let-7j | 1 | UGAGGUAGUUGUUUGUACAGUU | 22 | dre | |
| pol-miR-9* | pol-miR-9* | 3 | UAAAGCUAGAUAACCGAAAGU | 21 | mdo, xtr, dre |
| pol-miR-10 | pol-miR-10b | 1 | UACCCUGUAGAACCGAAUUUGU | 22 | oan, xtr, ppa, mne, ggo, gga, omy |
| pol-miR-21 | pol-miR-21a | 1 | UAGCUUAUCAGACUGGUGUUGG | 22 | omy |
| pol-miR-23 | pol-miR-23a | 3 | AUCACAUUGCCAGGGAUUUCCA | 22 | oan, bta, tni, fru, dre |
| pol-miR-26 | pol-miR-26a | 1 | UUCAAGUAAUCCAGGAUAGGCU | 22 | eca, cfa, bta, oan, tni, fru, ppa, mml, mne, lla, ppy, ggo, ptr, ssc, dre, rno, mmu, hsa, omy |
| pol-miR-125 | pol-miR-125b | 1 | UCCCUGAGACCCUAACUUGUGA | 22 | eca, bfl, spu, sko, cap, bfl, dya, dwi, dvi, dsi, dse, dpe, dmo, dgr, der, dan, tca, cfa, oan, mdo, xtr, bta, tni, fru, lca, mne, lla, sla, mml, ptr, ppy, ppa, age, ggo, ssc, dre, aga, dps, gga, rno, hsa, dme, mmu |
| pol-miR-128 | pol-miR-128 | 2 | UCACAGUGAACCGGUCUCUUU | 21 | eca, cfa, mml, oan, mdo, bta, ptr, gga, rno, hsa, ppa, mmu, omy |
| pol-miR-145 | pol-miR-145 | 1 | GUCCAGUUUUCCCAGGAAUCCC | 22 | dre, omy |
| pol-miR-181 | pol-miR-181a | 1 | AACAUUCAACGCUGUCGGUGAGU | 23 | eca, oan, mdo, xtr, tni, fru, lla, ppy, mne, sla, mml, ptr, ppa, ggo, dre, gga, rno, hsa, mmu, omy |
| pol-miR-181f | 1 | AACAUUCAACGCUGUCGGUGAGUU | 24 | bta | |
| pol-miR-200 | pol-miR-200a | 1 | UAACACUGUCUGGUAACGAUGU | 22 | eca, ptr, mml, mdo, xtr, tni, fru, dre, gga, rno, hsa, mmu |
| pol-miR-221 | pol-miR-221 | 1 | AGCUACAUUGUCUGCUGGGUUUC | 23 | eca, ptr, mdo, xtr, tni, fru, ppa, ppy, ggo, mml, dre, gga, rno, mmu, hsa |
| pol-miR-429 | pol-miR-429 | 3 | UAAUACUGUCUGGUAAUGCCGU | 22 | bta, oan, xtr, gga, tni, fru, dre, cfa, rno, mmu |
| pol-miR-724 | pol-miR-724 | 1 | UUAAAGGGAAUUUGCGACUGUU | 22 | dre |