Table 1.
Summary of strains and plasmids used in this work.
| Plasmids | Genotype | Remarks |
|---|---|---|
| pCM655/ DapE |
Plasmid with dapE gene | Allows overexpression of DapE, thus rescuing DapE knockout strains (even in absence of mDAP supplementation). Also confers ampicillin-resistance |
| pCM699 | Plasmid without dapE gene | Confers ampicillin-resistance, but does not genetically rescue DapE knockout strains |
|
| ||
| S. enterica strains | ||
| TN5911 | dapE knockout strain | Strain does not grow in absence of mDAP supplementation |
| TN5935 | TN5911 + pCM655/DapE | DapE is overexpressed from plasmid and strain can grow in absence of mDAP supplementation |
| TN5959 | TN5911 + pCM699 | Plasmid does not contain DapE and knockout strain does not grow in absence of mDAP supplementation |
|
| ||
| E. coli strains | ||
| XL1-Blue | Wildtype (from Stratagene) | Has endogenous DapE and can grow in absence of mDAP supplementation |
| TN5960 | XL1-Blue + pCM699 | Plasmid does not contain DapE, but strain can grow in absence of mDAP supplementation due to endogenous DapE |
| TN5962 | XL1-Blue + pCM655/DapE | In addition to endogenous DapE, this strain has plasmid-encoded DapE overexpression (from S. enterica). |