Table 2.
Aluminum regulated-proteins detected in soybean cultivar Young in time-course Al stress Experiment.
| Spot | Accession | Protein ID/functional classa | Average expression fold changeb | |||||
|---|---|---|---|---|---|---|---|---|
| 6 h | SE | 51 h | SE | 72 h | SE | |||
| 3 | gi/27883937 | Phosphoribosylformayl glycinamidine synthase (PM) | −1.34 | 0.13 | 1.33 | 0.10 | 1.45 | 0.20 |
| 52 | gi/224160640 | Zinc Knuckle (UN) | 1.24 | 0.18 | nd | 0.17 | 1.67 | 0.17 |
| 87 | gi/11385431 | Glutathione (DD) | nd | — | 1.22 | 0.08 | 4.34 | 1.04 |
| 100 | gi/156938901 | Profilin/actin binding (CS) | 1.92 | 0.8 | nd | — | nd | — |
| 103 | gi/79321519 | Heat shock protein 70 (PD) | −1.02 | 1.10 | 1.08 | 1.10 | 1.44 | 0.06 |
| 104 | gi/153805634 | Unknown (UN) | nd | — | nd | — | 1.38 | 0 |
| 106 | gi/15219879 | Apoptotic ATpase (DD) | −1.08 | 0.01 | 1.15 | 0.11 | 1.44 | 0.10 |
| 107 | gi/30696901 | Xylose isomerase (PM) | 1.05 | 0.01 | nd | — | 1.37 | 0.09 |
| 109 | gi/168030956 | Serine/threonine∖protein kinase (ST) | nd | — | nd | — | 1.31 | 0.04 |
| 112 | gi/436169 | Lipoxygenase (PM) | −1.10 | 0.03 | 1.13 | 0.05 | 1.35 | 0.23 |
| 113 | gi/77552532 | Unknown (UN) | −1.52 | 0.62 | 1.21 | 0.06 | 1.38 | 0.18 |
| 116 | gi/2232254 | NADH:flavin oxidoreductase (UN) | −1.36 | 0.06 | nd | 1.49 | 0.18 | |
| 118 | gi/223541365 | Transitional endoplasmic reticulum ATPase (CS) | nd | — | 1.37 | 0.03 | 1.37 | 0.27 |
| 119 | gi/223974443 | Unknown (UN) | −1.05 | 0.01 | −1.03 | 1.07 | 1.31 | 0 |
| 133 | gi/42820320 | Copper amino oxidase (DD) | 1.03 | 1.05 | nd | — | 1.35 | 0.07 |
| 136 | gi/195636596 | T-complex protein 1 alpha (PD) | 1.06 | 0.02 | 1.35 | 0 | 1.05 | 1.37 |
| 137 | gi/125574688 | Glutathione-s-transferase (DD) | 1.19 | 0.17 | 1.21 | 0.08 | 1.33 | 0.35 |
| 140 | gi/74053562 | Tubulin beta-3-chain (CS) | −1.03 | 0.01 | 1.02 | 0.08 | 1.62 | 0.3 |
| 147 | gi/5225811 | Unknown (UN) | 1.03 | 0.03 | 1.05 | 1.06 | −1.39 | 0.01 |
| 151 | gi/124112056 | Septum site determining protein (CS) | nd | — | 1.52 | 0.53 | −1.36 | 0.05 |
| 155 | gi/87240711 | V-ATPase subunit C (DD) | nd | — | nd | — | 1.31 | 0.23 |
| 156 | gi/1346698 | Phosphoglycerate kinase (PM) | −1.03 | 0 | 1.14 | 1.20 | 1.45 | 0.54 |
| 157 | gi/1582580 | Caffeic acid O-methyl transferase (SM) | −1.04 | 1.06 | nd | — | 1.35 | 0.09 |
| 158 | gi/1346028 | Polyprenyl synthetase (SM) | 1.09 | 1.09 | 1.06 | 0.04 | 1.32 | 0.05 |
| 166 | gi/3023197 | 14-3-like (ST) | nd | — | 1.09 | 0.04 | −1.32 | 0.23 |
| 167 | gi/393401 | Alpha tubulin (CS) | 1.21 | 0 | 1.41 | 0.06 | −1.42 | 0.20 |
| 172 | gi/78146198 | MADS box protein/transcription factor (TF) | 1.11 | 0.11 | nd | — | 1.85 | 0.74 |
| 185 | gi/115511406 | Acirduyctase dioxygenase (PM) | 1.25 | 0.13 | nd | — | 1.61 | 0.01 |
| 186 | gi/224105487 | Unknown (UN) | 1.11 | 1.22 | 1.22 | 0.21 | 1.34 | 0 |
| 187 | gi/145352433 | Unknown (UN) | −1.01 | 1.02 | 1.44 | 0.01 | 1.59 | 0.25 |
| 188 | gi/17380185 | Proteasome subunit beta type-1 (PD) | 1.04 | 0.02 | nd | — | 1.72 | 0.11 |
| 191 | gi/223543735 | Proteasome subunit beta type (PD) | −1.04 | 0.02 | nd | — | 1.74 | 0.52 |
| 193 | gi/145340582 | Unknown (UN) | 1.08 | 0 | nd | — | 1.41 | 0.08 |
| 195 | gi/209778987 | Putative rab1C protein (IT) | −1.05 | 1.15 | −1.02 | 1.10 | −1.67 | 0.29 |
| 196 | gi/18395025 | 20S proteasome beta subunit C (PD) | 1.07 | 1.16 | nd | — | 1.32 | 0.27 |
| 197 | gi/125577605 | DNJ/HSP40 (DD) | 1.04 | 1.16 | 1.00 | 1.07 | 1.35 | 0.31 |
| 198 | gi/34485411 | Resistance protein (DD) | 1.00 | 1 | 1.40 | 0.06 | 1.73 | 0.37 |
| 200 | gi/20140683 | Translationally-controlled tumor protein homolog (CD) | nd | — | −1.53 | 0.08 | −1.46 | 0.10 |
| 201 | gi/40644130 | Allene oxide cyclase (PM) | −1.51 | 0.47 | nd | — | 1.59 | 0.26 |
| 204 | gi/223510245 | Unknown (UN) | 1.04 | 0.04 | 1.01 | 1.17 | 1.32 | 0.05 |
| 206 | gi/197294157 | Hypothetical protein (UN) | −1.53 | 0.55 | nd | — | 1.42 | 0.22 |
| 212 | gi/110931690 | MYB transcription factor (TF) | 1.39 | 0.38 | −1.93 | 0.75 | nd | — |
| 214 | gi/18143656 | Mcp20 (PD) | 1.35 | 0.18 | nd | — | 1.61 | 0.73 |
| 218 | gi/829282 | Eukaryotic initiation factor A (PS) | 1.93 | 0.46 | nd | — | nd | — |
| 220 | gi/15238219 | Unknown (UN) | nd | — | −1.32 | 0.08 | −1.72 | 0.08 |
| 225 | gi/223547693 | Electron transporter (E) | nd | — | −1.02 | 1.07 | 2.10 | 0.20 |
| 226 | gi/145345251 | Glycogen synthase kinase-3 (PM) | nd | — | nd | — | 1.77 | 0.21 |
aPM: primary metabolism, SM: secondary metabolism, E: energy, PD: protein destination, TF: transcription factor, CS: cell structure, ST: signal transduction, DD: disease/defense, t, IT: intracellular trafficking, CD: cell division, UN: unknown function;
bexpression fold change at each time point, negative values: downregulation, positive values: upregulation, fold change significance cut-off is ±1.3, SE: standard error, nd: not detected.