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. 2011 Mar 1;3(2):48–57. doi: 10.4161/isl.3.2.15016

Figure 3.

Figure 3

Glucose-, but not KCl-stimulated insulin secretion, is attenuated in INS 832/13 cells following siRNA-mediated knockdown of ICMT. INS 832/13 cells were either mock transfected or transfected with scrambled siRNA or siRNA-ICMT at a final concentration of 100 nM and cultured for 24 h. Transfection efficiency was determined by separating equal amounts of proteins on SDS-PAGE and probing with ICMT antibody [(A) representative of three transfections is shown here]. Data in (A) was densitometrically analyzed and expressed as fold change over basal (B). *p < 0.05 compared with mock or scrambled siRNA transfected cells. Further, transfected cells were incubated either with low glucose (LG; 2.5 mM) or high glucose [(C) HG; 20 mM] or a membrane depolarizing concentration of KCl [(D) 60 mM; osmolaity adjusted by lowering NaCl] for 45 min at 37°C. Insulin released into the medium was quantitated by ELISA. Data are expressed as percentage of basal and are mean ′ SEM from three independent determinations. *p < 0.05 vs. respective low glucose controls; **p < 0.05 vs. mock transfected cells.