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. 2011 Mar 1;3(2):48–57. doi: 10.4161/isl.3.2.15016

Figure 4.

Figure 4

Depletion of endogenous ICMT markedly attenuates glucose-induced activation of Rac1 in INS 832/13 cells. INS 832/13 cells were transfected with ICMT-siRNA or mock transfected and cultured for 24 h. At confluence, cells were starved overnight and stimulated with either low (2.5 mM) or high (20 mM) glucose for 30 min. The extent of Rac1 activation in these cells was quantitated by PAK-PBD pulldown assay. Total and activated (Rac1.GTP) were determined by western blotting (A) and quantitated by densitometry (B). Data are expressed as fold change in Rac1 activation and are mean ± SEM from three independent determinations. *p < 0.05 vs. mock transfected low glucose; **p < 0.05 vs. mock transfected high glucose.