Figure 5.

Glucose-induced ROS generation was attenuated in INS 832/13 cells following siRNA-mediated knockdown of ICMT. INS 832/13 cells transfected with ICMT-siRNA (or mock transfected) following which cells were stimulated with low glucose (2.5 mM) or high glucose (20 mM) for 1 h and were incubated with DCHFDA (10 µM; 30 min) and harvested for quantitation of DCF fluorescence. Data expressed as DCF fluorescence and are mean ± SEM from three independent determinations. *p < 0.05 vs. respective low glucose; **p < 0.05 vs. high glucose in mock transfected cells.