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. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Growth Horm IGF Res. 2005 Mar 23;15(3):207–214. doi: 10.1016/j.ghir.2005.02.008

Fig. 1.

Fig. 1

RhIGFBP-3 induced apoptosis in RMC. FACS analysis: The cells were treated with or without 2 μg/mL IGFBP-3 in SFM containing 5 mM glucose for 2 h (a) or 24 h (b). Black lines represent rhIGFBP-3 treatment, and blue lines represent SF control. Annexin (upper panel) reflects apoptosis, and propidium iodide stain (lower panel) depicts necrosis. DNA fragmentation ELISA: (c) cells were treated with or without 2 μg/mL rhIGFBP-3 under 5 or 25 mM glucose-containing media for 24 h. Data were represented as means ± SE (n = 8; *p < 0.05 vs. SFM control by ANOVA).