Figure 2.

Evaluation of cleaning conditions. Different cleaning conditions were evaluated by incubation of the fouled MabSelect SuRe resin in cleaning chemicals and sequences of cleaning steps. An incubation time of 15 min was used, which corresponds to the CIP contact time in a column. Between each cleaning step in a sequence, the resin was washed with 300 µL PBS followed by wash with 300 µL H₂O. In each step, mixing was done and centrifugation was used for liquid removal between each step.