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. 2011 Mar 1;3(2):192–202. doi: 10.4161/mabs.3.2.14874

Figure 3.

Figure 3

Reduced SDS-PAGE (Deep Purple™ stained) for analysis of proteins remaining on the artificially fouled MabSelect SuRe resin after cleaning with different agents. The control sample, which was washed with PBS (lane 1), showed that there was a large amount of proteins on the artificially fouled resin. NaOH proved to be very effective in cleaning, and increasing NaOH-concentration resulted in increasing cleaning efficiency (lanes 2–4). 0.1 M NaOH + 1.0 M NaCl (lane 7) resulted in a significant decrease in cleaning efficiency compared to 0.1 M NaOH without salt (lane 2).