Figure 6.

Schematic description of fouling of MabSelect SuRe resin in PreDictor plates by repeated bind-elute cycles. Steps 1 and 3 show equilibration or wash with PBS (20 mM phosphate, 0.15 M NaCl, pH 7.4). In step 2, harvested cell culture fluid with mAb was added to the resin and incubation was done for 30 min. In step 4, the bound mAb was eluted with 0.1 M sodium citrate pH 3.5. Steps 1–4 were repeated ten times, corresponding to ten chromatography cycles. In each step, mixing was done and centrifugation was used for liquid removal between each step.