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. 2011 Mar 1;3(2):192–202. doi: 10.4161/mabs.3.2.14874

Figure 8.

Figure 8

Remaining impurities on fouled MabSelect SuRe resin after cleaning with 32 different protocols including one control (n = 3, mean ± SD). Detailed information about the composition of the CIP protocols is found in Table 1. Low protein concentration on the resin corresponded to efficient cleaning and vice versa. CIP protocol 11 is cleaning with 0.1 M NaOH. In CIP protocols 12–20, 0.1 M NaOH was combined with acidic steps, solvent, alcohol or chaotropic agents. CIP 21–23 corresponded to 0.3–0.5 M NaOH with/without acidic cleaning. In CIP 24–29 the resin was cleaned with reducing agent followed by 0.1 M NaOH with or without acidic cleaning and CIP 30 and 31, reducing agent was followed by 0.5 M NaOH. In CIP 32, the reversed order, i.e., 0.1 M NaOH followed by 100 mM 1-thioglycerol was used.