Table 1.

Effect of biocatalyst load on synthesis of isopropyl acetate in n-heptane by silica-bound lipase. The reaction mixture containing 100 mM isopropanol and 75 mM acetic acid and silica-bound biocatalyst in n-heptane was incubated at 55°C under shaking for 9 h.

Immobilized enzyme (mg)	Ester synthesis (mM)
25	67 ± 0.01
50	66 ± 0.01
75	65 ± 0.02
100	63 ± 0.01
125	62 ± 0.02