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. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 13;386(4):554–558. doi: 10.1016/j.bbrc.2009.06.045

Fig. 1. Mouse 2410018M08Rik/Scand3 gene.

Fig. 1

A. Diagram of the exon-intron organization of the 2410018M08Rik/Scand3 gene and conservative domains of the Scand3 protein. The exons are shown as boxes and drawn to scale (scale bar shown), and the introns, which vary greatly in size, are represented in a uniform manner. CHCH - coiled-coil-helix-coiled-coil-helix motif (pfam06747); hATC - hAT family dimerisation motif (pfam05699).

B. Transcription factor binding sites in the proximal promoter of Scand3. The arrow indicates the putative transcription start site. Sequences are shown for the TFII-I-binding consensus BRGATTRBR; the core part is boxed. The relative positions are shown for the following consensus sequences: CRE — cyclic AMP response element (TGASSTCA); NFkB — binding site for activated NFkB complex; p300 - p300 binding site RGGAGT [14]; SMAD - the SMAD family binding site (CAGACA); STAT - STAT binding site (TTCNNNGAA). The transcription factor binding sites (TFBS) on plus-strand are shown above each of the diagrams and TFBS on minus-strand are indicated below the diagrams.

C. The time-course of Nanog, Pou5f1(Oct3/4), and Scand3 expression in mouse R1 embryonic stem cells differentiating into embryoid bodies in vitro (GDS2667 record). The error bars represent standard deviation calculated from three independent arrays.