Figure 3. Semi-quantitative PMCA to assess RML PrP^TSE degradation.

(A) The extent of mouse RML PrP^TSE degradation was measured by PMCA analysis of dilutions (10⁻²–10⁻⁶) of vehicle or L. pulmonaria extract-treated RML. (B) Control reactions lacking PrP^TSE seed or sonication did not show amplification as measured by PK digestion. (C) RML samples were incubated with vehicle for 1 hr or with L. pulmonaria extract (reacted: 1 hr; spiked <10 sec) then diluted and subjected to PMCA. Adding L. pulmonaria extract in PMCA reactions without allowing time for reaction (spiked samples) does not appear to reduce PrP^TSE levels or affect amplification compared to control (vehicle). All immunoblots used mAb SAF83.