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. 2011 May 11;6(5):e19718. doi: 10.1371/journal.pone.0019718

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Huang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Recombinant His-tagged Aurora-A or kinase-inactive Aurora-A mutant protein (K162I) was incubated with the indicated amount of GST-PUM2 for 20 min at 30°C in kinase reaction buffer containing histone-H3 and ATP. The activity of Aurora-A was detected by immunoblotting using the anti-phospho-Aurora-A-T288 and the anti-phospho-Histone-H3 antibodies.