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. Author manuscript; available in PMC: 2012 May 17.
Published in final edited form as: Biochemistry. 2011 Apr 25;50(19):3913–3918. doi: 10.1021/bi2002554

Figure 1.

Figure 1

Result of a gel-shift assay following incubation of 0.2 μM of a 171bp-DNA fragment containing (a) a perfectly-matched (PM, lanes 1–5) and single-base mismatched (MM, lanes 6 and 7) binding site with 1.0 μM of the indicated γPNA oligomers, and (b) a PM binding site with the indicated concentrations of PNA1-3X oligomer in a simulated physiological buffer at 37 °C for 16 hr, followed by separation on nondenaturing PAGE and staining with SYBR-Gold.