Skip to main content
. Author manuscript; available in PMC: 2012 May 17.
Published in final edited form as: Biochemistry. 2011 Apr 25;50(19):3913–3918. doi: 10.1021/bi2002554

Figure 4.

Figure 4

Effects of temperature and incubation-time on DNA strand invasion by PNA1-3X. The fraction bound was determined by gel-shift assays following incubation of 0.2 μM of DNA containing a PM binding site with 1.0 μM of PNA1-3X in a simulated physiological buffer at the indicated temperatures and time-points. t1/2 is defined as the time it took to reach 50% binding. Inset: A plot of −ln(1−C) vs. incubation time; the data shows that strand of invasion of B-DNA by PNA1-3X at 37 °C obeys pseudofirst-order kinetics: C = 1 − exp(−kpst), where C is the fraction bound at time t and kps is the pseudofirst-order rate constant (kps, 37 °C = 0.0075 min−1).