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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Nano Lett. 2011 Apr 20;11(5):2178–2182. doi: 10.1021/nl200862n

Figure 5. Uptake Kinetics and Excess Polymer.

Figure 5

(a) Percentage of positive cells was monitored for 5 hr, beyond a typical length of transfection time. Larger nanocomplexes from bulk preparation initiated with a higher uptake at early time point. As nanocomplexes aggregated, their uptake reached a plateau. In contrast, small and stable MAC prepared nanocomplexes were favorable for a consistent uptake. (b) Because the amount of genetic materials in different size of nanocomplexes varied, geometric mean fluorescence may be a preferable indication of total uptake. (c) Significantly lower excess polymer remained in the MAC preparation, whereas ~70% of polymer at a ratio of 2μL Turbofect transfection reagent per μg of DNA was unreacted in the bulk preparation. Mean ± s.e.m. (n = 3).