Fig 7.

CM protects cardiac myocytes from apoptotic death and promotes myocyte hypertrophy. The direct effects of control medium, CM + mouse IgG isotype control (mIgG) or CM +αIL-6 MAb on neonatal rat cardiac myocytes were examined. (A) Hypoxia-induced apoptosis was determined by TUNEL (brown). Blue: viable nuclei. Myocyte hypertrophy was determined by measuring total protein content, relative to DNA content (B) and phalloidin staining of fixed cells (C). Data are represented as mean ± S.E.M. Each condition was represented in triplicate. N= 3 experiments. *P < 0.05.