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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Dev Dyn. 2011 Apr 12;240(6):1467–1474. doi: 10.1002/dvdy.22642

Figure 5.

Figure 5

Dox-induced dpTR caused changes in gene expression. Tadpoles doubly transgenic for the two constructs shown were treated with and without 50 ug/mL Dox beginning at NF stage 52–54 for three days. A tadpole singly transgenic for pDRTREdpTR-HS4 was similarly treated with Dox and used as a control. Tails were harvested for the analysis. Each lane of the gel represents an individual tadpole. The presence of Dox upregulated the transgene dpTR, which in turn induced expression of the two thyroid hormone response genes, thyroid hormone receptor beta (TRβ) and thyroid hormone-induced basic leucine zipper (bZIP). Consistent expression of the control gene ribosomal protein L8 (rpL8) in each lane indicates comparable sample quality.