Table 1.
Prediction of partial zinc binding sites in FH
| a. Distribution of predicted zinc coordination sites in 20 SCR domains | ||
|---|---|---|
| Domain | Structure | Sitesa |
| SCR-1 | 2RLP | 6 |
| SCR-2 | 2RLP, 2RLQ , 2RLQ | 14.5 |
| SCR-3 | 2RLQ | 23.5 |
| SCR-4 | Homology | 2 |
| SCR-5 | NMR structure | 0 |
| SCR-6 | 2UWN | 62.5 |
| SCR-7 | 2UWN | 8.5 |
| SCR-8 | 2UWN | 1 |
| SCR-9 | Homology | 5 |
| SCR-10 | Homology | 4 |
| SCR-11 | Homology | 14 |
| SCR-12 | Homology | 9 |
| SCR-13 | Homology | 8 |
| SCR-14 | Homology | 4 |
| SCR-15 | 1HFH | 7.5 |
| SCR-16 | 1HFH | 19.5 |
| SCR-17 | Homology | 0 |
| SCR-18 | Homology | 1 |
| SCR-19 | 2G7I | 7.5 |
| SCR-20 | 2G7I | 4.5 |
| b. Possible predicted zinc binding sites in the FH fragment SCR-6/8b | ||
|---|---|---|
| Residues | Location | Possible zinc coordination |
| D326–D329 | SCR-6 | DDH, DDHH |
| D329–H332 | SCR-6 | DDHH, DHHH |
| H332–D358 | SCR-6 | DDDH, DDHH, DEHH, DH, DHH, DHHH |
| H332–D358–E359 | SCR-6 | DEHH |
| H332–D358–H360 | SCR-6 | DDHH, DEHH, DHH, DHHH |
| H332–D358–E362 | SCR-6 | DDHH, DEHH |
| H332–E359 | SCR-6 | EH, EHH, EHHH |
| H332–H360 | SCR-6 | DDHH, DEHH, DHH, DHHH, EHH, HH, HHH |
| H332–H360–E362 | SCR-6 | DEHH |
| H332–H360–E433 | SCR-6/7 | DEHH |
| H332–E362 | SCR-6 | DEHH, EHH, EHHH |
| H332–E433 | SCR-6/7 | EHHH, DEH, DEHH |
| H337–E338 | SCR-6 | EH, EHHH |
| D358–E359 | SCR-6 | DE |
| D358–H360 | SCR-6 | DDHH, DH, DHH, DHHH |
| D358–H360–E362 | SCR-6 | DEHH |
| H360–E362 | SCR-6 | DEHH, EEEH, EEHH, EH, EHHH |
| H360–E433 | SCR-6/7 | EHH, DEHH, EHHH |
| D370–H371 | SCR-6 | DDHH, DEHH, DHH |
| D370–H371–H373 | SCR-6 | DDHH, DHH, DHHH |
| D370–H373 | SCR-6 | DDHH, DHH, DHHH |
| H371–H373 | SCR-6 | DHHH, EEHH, EHH, HHH, HHHH |
| H373–D377 | SCR-6 | DHHH, DDHH |
| E395–H402 | SCR-7 | EHH, EHHH |
| E395–H417 | SCR-7 | DEHH, EHH, EHHH |
| D454–E456 | SCR-8 | DDH, DDE |
a
Zinc coordination sites that are shared between two adjacent SCR domains are counted as 0.5 per domain.
b
His402 occurred in 2 of the 76 possible zinc coordinations in SCR-6/8. Zinc coordinations that contain two or three residues also contain two or one H2O molecules, respectively, to form the required tetrahedral geometry with zinc.