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. Author manuscript; available in PMC: 2011 May 12.
Published in final edited form as: Free Radic Biol Med. 2009 Jan 15;46(8):1042–1048. doi: 10.1016/j.freeradbiomed.2008.12.024

Figure 1. Lon is induced with hydrogen peroxide treatment.

Figure 1

A, Lon protein analysis of RD cells treated with 0, 100, 200, or 400μM H2O2 for 1 hour and then left to recover for up to 25 hours after H2O2 treatment. ‘No H2O2’ represents samples that were incubated with vehicle instead of hydrogen peroxide. Blots were probed with anti-Lon antibody or anti-porin antibody as a mitochondrial loading control. The bar graph represents data normalized against porin. B, mRNA analysis by qRT-PCR of the same samples examined in A. Samples were amplified with Lon and GAPDH primers and calculation of Lon mRNA levels were normalized against GAPDH as an internal loading control. In both panels A and B, all data points shown are the means ± standard errors of three independent determinations.