Table 1.
Classification of regeneration defects observed in Rx shRNA transgenic embryos.
| Classification | Regeneration defect | Regeneration phenotype | Non-transgenic control1 | Control shRNA2 | Rx shRNA3 | Rescue4 |
|---|---|---|---|---|---|---|
| Category 1 | No defect | Elongated RPCs that span all retinal layers at the wound site | 21/22 95.4% |
16/16 100% |
7/25 28% |
7/13 53.8% |
| Category 2 | Defective RPCs | RPCs are shorter and/or rounder and sometimes disorganized at wound site | 0/22 0% |
0/16 0% |
13/25 52% |
5/13 38.4% |
| Category 3 | Defective RPCs and RPE | RPCs are defective as described above and the RPE is disorganized; not completely reformed around the wound | 1/22 4.5% |
0/16 0% |
5/25 20% |
1/13 7.7% |
Morphological defects in retinal regeneration were scored at 9 days post-resection and the defects were assigned to each category
1
Non-transgenic control-wild type tadpoles
2
Control shRNA - tadpoles transgenic for the control shRNA
3
Rx shRNA- tadpoles transgenic for the Rx shRNA
4
Rescue- tadpoles co-transgenic for the Rx shRNA and the mRx transgene