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. 2011 May 15;22(10):1677–1685. doi: 10.1091/mbc.E10-08-0677

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© 2011 Capaldo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Cyclin D1 cosediments with TJs in polarized epithelial cells. (A) Representative Western blot of cyclin D1 in isolated SKCO-15 cytosolic, cell membrane, and nuclear fractions. Tubulin is shown as a marker of cytosolic protein and lamin A/C as a nuclear marker. (B) Densitometry analysis of Western blot; n = 3. (C) Sucrose density gradient fractionation of SKCO-15 cell lysate showing cosedimentation of cyclin D1 with tight junction proteins. (D) Sucrose density of indicated fractions (15–60%; red) and ALP activity in the corresponding fractions (blue; n = 8).