FIGURE 3:

Pex34p acts by itself and together with the proteins of the Pex11 family to control peroxisome size and number under conditions of peroxisome proliferation. (A) β-galactosidase filter assay to test for interaction between Pex34p and Pex34p, Pex11p, Pex25p, Pex27p, Fis1p, and Vps1p by yeast two-hybrid analysis. Two independent transformants for each strain are shown. (B) Wild-type BY4742 cells and cells of the pex34Δ, pex11Δ, pex25Δ, pex27Δ, pex34Δpex11Δ, pex34Δpex25Δ, and pex34Δpex27Δ deletion strains expressing Pot1p-GFP were grown for 16 h in oleic acid–containing SCIM and imaged by confocal fluorescence microscopy. Bar, 5 μm. (C) Cells were scored for the number of Pot1p-GFP–labeled punctae per cell. Graphic results represent the average number of punctae ± SEM of three independent experiments and 20 cells per experiment.