TABLE 1:
Ability of UNC-84 domain deletion transgenic constructs to rescue nuclear migration.
| UNC-84 constructa | Line | Number of nuclei in dorsal cordb |
|---|---|---|
| None (wild-type background) | N2 | 0 ± 0 (11) |
| None (unc-84(n369) background) | MT369 | 14.3 ± 1.5 (25) |
| UNC-84 (+) rescuing construct | UD87 | 0.2 ± 0.5 (25) |
| UNC-84ΔSUN | UD61 | 14.8 ± 1.1 (≥15)c |
| Transmembrane domain analysis (Figure 2) | ||
| ΔH1 (386–406) | 1 | 0.2 ± 0.5 (20) |
| 2 | 0.8 ± 1.9 (10) | |
| ΔH2 (512–532) | 1 | 14.5 ± 1.6 (26) |
| 2 | 14.1 ± 1.5 (17) | |
| 3 | 13.6 ± 1.2 (13) | |
| 4 | 14.5 ± 1.4 (12) | |
| ΔH3 (685–705) | 1 | 1.4 ± 3.4 (25) |
| 2 | 2.6 ± 4.8 (16) | |
| ΔH5 (719–738) | 1 | 0.8 ± 2.2 (44) |
| 2 | 1.5 ± 4.1 (10) | |
| ΔH3–5 (685–782) | 1 | 0.7 ± 2.1 (19) |
| 2 | 1.5 ± 2.3 (11) | |
| 3 | 2.4 ± 2.3 (10) | |
| ΔH1,3–5 (386–406;685–782) | 1 | 2.2 ± 4.3 (6) |
| NLS analysis (Figure 6) | ||
| 2xcNLS (KK 38–39 AA; HRRR 170–173 AAAA) | 1 | 4.8 ± 3.6 (13) |
| 2 | 1.3 ± 1.9 (10) | |
| 3 | 2.9 ± 2.5 (8) | |
| INM-SM (KKSSK 503 AASSA) | 1 | 0.5 ± 1.9 (33) |
| 2 | 0.6 ± 1.8 (8) | |
| 2xcNLS; INM-SM | 1 | 0.7 ± 1.7 (13) |
| ΔSUN-NELS (235–240) | 1 | 0.7 ± 1.7 (9) |
| 2 | 0.1 ± 0.3 (9) | |
| 3 | 0.9 ± 1.9 (7) | |
| ΔSUN-NELS (226–251) | 1 | 1.1 ± 1.8 (14) |
| ΔSUN-NELS (235–240); INM-SM | 1 | 0.9 ± 2.9 (15) |
| 2 | 0.5 ± 1.8 (11) | |
| ΔSUN-NELS (226–251); INM-SM | 1 | 2.9 ± 3.2 (23) |
| 2 | 5.2 ± 3.3 (16) | |
| 3 | 1.8 ± 3.4 (12) | |
| 2xcNLS; ΔSUN-NELS (235–240) | 1 | 13.0 ± 2.0 (66) |
| 2 | 13.7 ± 1.9 (31) | |
| 3 | 13.6 ± 1.4 (17) | |
| 2xcNLS; ΔSUN-NELS (226–251); INM-SM | 1 | 14.0 ± 1.2 (23) |
| 2 | 12.3 ± 3.1 (10) | |
| 3 | 14.4 ± 0.9 (8) | |
| 4 | 13.4 ± 2.3 (7) | |
| 5 | 12.8 ± 1.5 (6) | |
| 6 | 14.5 ± 0.6 (4) |
aAll mutant constructs were constructed in the UNC-84 rescuing construct pSL38 (McGee et al., 2006). The exact residues deleted in the mutant constructs are indicated in parentheses.
bMean number of nuclei and the SD are shown. Sample size is in parentheses. Nuclei in dorsal cord were counted blindly, before determining whether the animal was transgenic. podr-1RFP or sur-5::GFP were used as transgenic markers. Nontransgenic animals in each blind count had an average of 13.3–15.3 nuclei (n is between 5 and 44). All lines are in an unc-84(n369) mutant background.
cFrom McGee et al., 2006.