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. 2011 May 12;7(5):e1001340. doi: 10.1371/journal.ppat.1001340

Figure 4. Cathepsins are essential for influenza A virus-induced inflammasome activation.

Figure 4

A. Human macrophages were left untreated or infected with influenza A virus for 6, 9, or 12 h, after which concentrated cell culture media was prepared for Western blot analysis with anti-cathepsin B, anti-cathepsin D, anti-caspase-1 Abs. B–C. Human macrophages were infected with influenza A virus in the presence and absence of 40 µM Ca-074 Me, after which the cell culture media was collected and analysed with IL-18 ELISA, and Western blot analysis with anti-IL-18 and caspase-1 specific Abs. D. Human macrophages were infected with influenza A virus in the presence and absence of Ca-074 Me (40 µM). After this total protein lysates were prepared and analyzed by Western blotting with anti-caspase-3 p19/17 and anti-H3N2 specific Abs.