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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Leukemia. 2011 Jan 28;25(5):792–799. doi: 10.1038/leu.2011.1

Figure 3.

Figure 3

Functional assessment of NK cell activity toward K562-CD200− and K562-CD200+ targets. (a) Summarized data illustrating percentage of degranulating NK cells in sub-populations 3, 4 and 5 (defined as in Figure 1a) in response to K562-CD200− or K562-CD200+ targets (Supplementary Figure S3). Background CD107a expression (PBMC control cultures with no targets) is also indicated. Flow cytometric gating strategy for this assay is shown in Supplementary Figure S4). (b) Representative flow cytometric plots illustrating expression of CD107a (open histograms) in sub-population 4 NK cells in response to K562-CD200− and K562-CD200+ targets, or PBMC control. Filled histograms represent isotype-matched controls. (c) IFNγ release in response to K562-CD200− targets compared with K562-CD200+ targets as measured by ELISPOT at different effector to target (E:T) ratios (see Materials and methods; representative data shown in Supplementary Figure S4). Data represent mean±1 s.d. (n=6, per group). *P<0.05 analyzed by one-way analysis of variance (ANOVA) with Turkey’s multiple comparison test.