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(A) Chromatogram of N run on an S200 size exclusion column. The grey line shows the absorbance at 280 nm, and the green line the absorbance at 260 nm. The inset shows a 12.5% SDS-PAGE gel of peaks 1 (N1) and 2 (N2). (B) A 12.5% SDS-PAGE gel showing the presence of dimers, trimers and tetramers after cross-linking fraction N2 with 0.05% glutaraldehyde for one hour at room temperature.
